Mango seed kernel oil (MSKO) contains a high concentration of polyphenol
and has potential as raw material of oil-based food products and as a natural antioxidant of functional food. Ethanol was used for the extraction of MSKO by
response surface methodology (RSM) as a tool to study the optimum extract-
ion conditions of the oil yield and total polyphenol content (TPC). Three-factor-
three-level central composite design (CCD) was employed to optimize extract-
ion time (X1), ethanol concentration (X2), and ethanol volume (X3) to obtain a high oil yield and TPC. The central points for treatment were 5 h for X1, 86% for X2, and 250 mL for X3. The results showed that the optimum conditions of
MSKO extraction were X1 = 5.18 h, X2 = 87.84% and X3 = 233.43 mL, res-
pectively. Under these conditions, the experimental oil yield and TPC of MSKO
were 34.79% and 61.17 mgGAE/g, which was agreed closely to the verification
value. The results indicated that MSKO extraction using ethanol could be an
effective and advisable method for large-scale production of MSKO extraction