GC-MS analysis and antibacterial activity of the Sea cucumber (Muelleria lecanora) extract

Muhammad, Yusuf and Nur Fitriani, Usdyana Attahmid (2021) GC-MS analysis and antibacterial activity of the Sea cucumber (Muelleria lecanora) extract. Journal of Biological Research, 94 (2). pp. 1-10. ISSN 2284-0230

[thumbnail of Artikel] Text (Artikel)
Lamp. B14_Artikel Invitro Antibacterial.pdf

Download (1MB)
[thumbnail of Turnitin] Text (Turnitin)
Turnitin In vitro antibacterial activity.pdf

Download (4MB)
[thumbnail of Korespondensi] Text (Korespondensi)
Lamp. B14_Korespondensi.pdf

Download (1MB)
[thumbnail of peer review] Text (peer review)
Hasil Peer Review (Sea Urchin).pdf

Download (2MB)


Sea cucumbers are marine invertebrates commonly found in
benthic areas and deep seas. On a global scale, they have a high commercial value with an increased level of production and trade. This study aims to analyze the bioactive compound in Sea cucumber (Muelleria lecanora) using the Ultrasound-Assisted Extraction Method (UAE) and Gas Chromatography-
MassSpectrometry (GC-MS). Furthermore, it identifies the antibacterial activityin microorganisms Salmonella, Escherichia coli, and Staphylococcus aureus. The bioactive compounds were extracted using methanol, acetone, and n-hexane solvent and were separated by ultrasound-assisted extraction. In the initial stage, phytochemicals were screened using Gas Chromatography-MassSpectrometry (GC-MS). Disc diffusion method was then used to determine theantibacterial activity against Salmonella, Staphylococcus aureus, and Escherichia coli. The results showed that methanol extract is more suitable for extracting bioactive compounds of Muelleria lecanora than acetone and n-hexane. Meanwhile, acetone solvents are more suitable for the production of flavonoid and steroid compounds than Mulleria lecanora samples. Heneicosane compounds that function as a new antiproliferative for inhibition of tumor and cancerous cells are produced from n-hexane. The antibacterial activity of acetone, methanol and n-hexane extract determined
by diffusion assay was effective against Staphylococcus aureus and Salmonella but ineffective againstEscherichia coli. GC-MS results showed that the major constituents obtained were steroid and flavanoid. From this study, Sea cucumber extract can be considered a healthy nutrientin food and pharmaceutical

Item Type: Article
Subjects: Q Science > QR Microbiology
T Technology > TP Chemical technology
Divisions: Jurusan Kimia > D4 Teknologi Kimia Industri
Depositing User: Muhammad Yusuf
Date Deposited: 23 Feb 2023 06:17
Last Modified: 23 Feb 2023 06:17
URI: http://repository.poliupg.ac.id/id/eprint/75

Actions (login required)

View Item
View Item