Optimization Ultrasonic Assisted Extraction (UAE) of Bioactive Compound and Antibacterial Potential from Sea Urchin (Diadema Setosum)

Muhammad Yusuf, Muhammad Yusuf and NUR FITRIANI USDYANA ATTHAMID, NUR FITRIANI USDYANA ATTHAMID and SRI INDRIATI, SRI INDRIATI and RAHMAWATI SALEH, RAHMAWATI SALEH and MAHYATI LATIEF, MAHYATI LATIEF and AKHMAD RIFAI, AKHMAD RIFAI Optimization Ultrasonic Assisted Extraction (UAE) of Bioactive Compound and Antibacterial Potential from Sea Urchin (Diadema Setosum). Current Research in Nutrition and Food Science, 8 (2). pp. 556-569. ISSN 2347-467X

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Abstract

Sea urchins can be used in medicine, has potential as a new type of antibiotic to developed in the pharmaceutical field, which is rich in bioactive compounds as a steroid, triterpenoids, saponins, and antioxidant properties. Conventional extraction generally takes a long time, is less environmentally friendly, and potentially triggers bioactive compound damage, so it needs alternative methods such as Ultrasound-Assisted Extraction (UAE). The extracting
technology, including ultrasonic-assisted extraction (UAE) and solvent variation (ethyl acetate and methanol) of bioactive compounds from sea urchin
(Diadema setosum) were optimized and compared. The purpose of this study was to study the application of UAE and solvent variation methods for sea
urchin extraction from the Barrang Lompo Island in South Sulawesi. Comparing
the maceration and ultrasonic assisted extraction methods. Optimization of
extraction with UAE was carried out on the variable of extraction duration and
type of solvent. Gas Chromatography-Mass Spectrometry results show that
ultrasound-assisted extraction generally produces compounds of CHOLEST-
5-EN-3-OL (3. BETA.), palmitic acid, 9-Octadecenoic acid (Z)-, methyl ester,
stearic acid, oleic acid, flavonoids, phenols, pentadecanoic acid and batilol
and streoid, which has a function as an antioxidant, anti-inflammatory,
anti-tumor, anti-cancer agents and antibacterial. The results showed the best
results using ultrasound-assisted extraction with a duration of 30 minutes and
using solvent ethyl acetate. These results implied that extracts obtained by
sonication showed the highest bioactive compounds and antioxidant activity,
thus proving that this activity depends directly on the antibacterial properties

Item Type: Article
Subjects: T Technology > TP Chemical technology
Divisions: Jurusan Kimia > D4 Teknologi Kimia Industri
Depositing User: Unnamed user with username 195901141988032001
Date Deposited: 30 Jul 2023 11:27
Last Modified: 30 Jul 2023 11:27
URI: http://repository.poliupg.ac.id/id/eprint/4550

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